2. the wedge smear 3. thespun smear. A small drop of blood is placed on the midline at the end of a glass slide. 2. ⇒ After 2 minutes, add twice the amount of distilled water or Phosphate buffer solution and mix the content by swirling or by blowing gently. Show ur Support, SUBSCRIBE! SMEAR PREPARATION 1. Wear gloves. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. and It is the most common smear preparation in the hematology laboratory and Wright stain, a Romanowsky stain, is the most common dye. If you continue browsing the site, you agree to the use of cookies on this website. BLOOD SMEAR EXAMINATION Making Blood smear MANUAL DIFFERENTIAL COUNTS These counts are done in the same area as WBC and platelet estimates with the red cells barely ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - … 1. Place another slide (spreader) with smooth edge at an angle of 30-45⁰ near the drop of blood. Thin blood film is prepared similarly to that of the differential white-cell count. You can change your ad preferences anytime. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. Clipping is a handy way to collect important slides you want to go back to later. The latter will prove useful if a problem oc-curs during the staining process and/or if you wish later to send the smears to a reference laboratory. Preparation involves placing a drop of well -mixed, ethylenediaminetetraacetic acid (EDTA) anticoagulated blood at one end of the slide. Meet Onco-life cancer centre's best hematologist in Pune, to clear all the doubts about Blood Clotting Disorders, visit Onco Life Cancer Centre. Now customize the name of a clipboard to store your clips. With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop. red blood cells, White blood cells & platelets, Biological membrane and transport BY Mohammadali, Customer Code: Creating a Company Customers Love, Be A Great Product Leader (Amplify, Oct 2019), No public clipboards found for this slide. (slide preparation and reporting) Blood Clotting Disorder | Hematologist in Pune - When a cut or injury occurs, the body stops the bleeding by forming a blood clot. See our User Agreement and Privacy Policy. BLOOD SMEAR PROCEDURE • Mix blood properly • Place a small drop of blood (about 2-3 mm) about 1 cm from the frosted end of a clean slide (slide must be completely clean) • Using a spreader (another slide), draw backwards into the drop of blood (while applying pressure) – maintain an angle of 30-45 degrees ⇒ If you don’t know how to prepare it, check out the preparation of Thick blood Smear. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. The are two additional types of blood smear used for specific purposes. Maintain an even gentle pressure on the slide. Precautions: Ensure that the whole drop of blood is picked up and spread. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. Selection of a spreader: Take one slide a spreader which has smooth edge. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. 1. Place a small drop of blood, or one side about 1-2 cm from one end. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. 3. Blood smear preparation and staining Prepared by: Ibtisam H. Al Aswad Amany S. Al Hindi Cytoplasm : pink Granules: primary secondary Nucleus: dark purple ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3ee768-MzBjN This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. The blood smear test or peripheral smear test is a type of blood test that is done to procure detailed information about the number and shape of blood cells.This test focuses on the red blood cells, white blood cells and platelets. It highlights the procedure to be followed and the equipment required. The most common technique of blood smear preparation is called the “wedge or push” technique. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. of collection) Preparation of Blood Film: The slide should be clean. Slide Method Procedure Place a drop of blood in the centre of a clean glass slide 1 to 2 cm from one end. red blood cells, White blood cells & platelets, Iron deficiency anemia pathogenesis and lab diagnosis, No public clipboards found for this slide. Move the spreader backward so that it makes contact with drop of blood. Blood smear is prepared, stained with Leishman’s stain and cells are identified under oil immersion lens. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count… This video describes the preparation of a peripheral blood smear. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. Prof. - Clinical Path 1/10/2013 Replaces 5/08 HEM 3.2.5 Procedure: Examination of the Peripheral Blood Smear (How to Perform) Preparation of Blood Smear: Collection of Sample: 1. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Preparing the smear requires attention to a number of details that help prevent contamination of the culture and ensure safety to the preparer. The preparation of a stained bacterial smear involves several steps. If you continue browsing the site, you agree to the use of cookies on this website. This ppt contain usefull information regarding different staining methods and stains with photographs...enjoy and learn it..give comments.. Looks like you’ve clipped this slide to already. PROCEDURE: A) Preparation of Blood Smear. The wedge blood smear will be discussed in this lab. Take one drop of venous or capillary blood. A poor slide is a torment. Samples with heparin are unusable for preparation of the smears. Dr. Ankur Patel Making and Staining a Blood Smear A well-made blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. All stages of P.vivax, P.ovale, and P.malariae can be seen in smear. Excessive washing may decolorize blood smear. The wedge smear is a convenient and commonly used technique for making peripheral blood … with different We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. View A385992265_12053_13_2018_Smear Preparation and staining.ppt from PHARMACY BTY304 at Lovely Professional University. Now customize the name of a clipboard to store your clips. Go to my Channel Page, click on "Videos". Blood smears are mostly done for Differential Leukocyte … sampling highly vascular masses, as blood contamination is often reduced. 4. Clipping is a handy way to collect important slides you want to go back to later. .Blood stored for more than four hours in anticoagulant before the preparation of blood smear can result in artifacts as red cell crenation and refractile border, also it may lead to degeneration of cellular elements of blood and may result in a pseudo-thrombocytopenia.2 Quality of the smear produced depends on proper smearing Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. COLLECTION TIPS AND ADVICE FOR CYTOLOGY SPECIMENS: 1. Peripheral blood smear examination The PowerPoint PPT presentation: "Preparation, staining and examination of blood … E.D.T.A. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. staining method Smear preparation techniques Direct smear Large volume centrifugation Small volume centrifugation Membrane filtration Cell block Density gradient centrifugation Gravity sedimentation Direct smear: A cytological smear made by spreading the specimen directly onto a glass slide.Direct smears are the simplest and most … Staining the Thick blood smear with Giemsa stain ⇒ Prepare a thick blood smear on a clean and dry microscopic glass slide and air dry it. This test provides information about the number and shape of these cells. The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. Making Thin Blood Smear: Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. | PowerPoint PPT … 1. 3. Keep the same angle all the way to the end of the smear. Smear preparation techniques 1. You can change your ad preferences anytime. Do not touch the surface of the slide where the blood smear will be made. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. However, the blood shouldn't clot when it's just moving through the body. Clean slides with 70 to 90% alcohol and allow to dry. SMEAR PREPARATION 1. Blood should be collected at or just after the height of febrile paroxysms; What stages of malarial parasites can be seen in peripheral blood smear. ⇒ Let the smear dry well in the air. 2. Finger Prick or . THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. See our Privacy Policy and User Agreement for details. from the ddacie and todd this ppt is there, for md pathology students. Staining Blood Smears . Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. 4. 4/10/2012 Approved Lisa Senzel Asst. blood (within 1 hr. 2. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. Peripheral Smear Preparation. A thin peripheral blood film is thus … Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. See our User Agreement and Privacy Policy. Preparation of blood smear ⇒Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. Then move the spreader forward rapidly over the slide. A smear can be prepared from a solid or broth medium. Ankurvety001@gmail.com. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the … What is the best time for collection of blood to demonstrate malarial parasite. 1. Looks like you’ve clipped this slide to already. Peripheral Smear Preparation Staining of Peripheral Blood Smear Peripheral Smear Examination OBJECTIVES 6. Check out my other Haematology videos! Giemsa stain - Recommended for detection and identification of blood parasites. Moderator- Dr. Namrata Shetty. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. If you continue browsing the site, you agree to the use of cookies on this website. Examination of the Peripheral Blood Smear.docPage 1 of 7 Printed 1/14/13 Created Lisa Senzel Asst. If you continue browsing the site, you agree to the use of cookies on this website. The drop should be in the center line of the glass slide. Making the peripheral blood smear. Capillary blood obtained by fingerstick: 1. 3. BLOOD SMEAR EXAMINATION I- Preparation of blood smear There are three types Wait until the blood spreads along the entire width of the spreader slide. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. Blood can also be taken from an EDTA sample. Once the needle is removed from the lesion, it is attached to an air-filled syringe and the material within the needle is gently expelled onto clean glass slide/s for smear preparation as described below. 2. See our Privacy Policy and User Agreement for details. Dr. Bahoran Singh Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. Stain only one set of smears, and leave the duplicates unstained. There are many reasons why your doctor may order a blood smear. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Cm from one end collect important slides you want to go back to later specific purposes this is... Of 30-45⁰ near the drop of blood film is prepared similarly to that of the slide on flat. Moving a thumb smoothly on its edge ( EDTA ) anticoagulated blood at one end of blood smear preparation ppt onto. One side about 1-2 cm from one end of slide date and time of ). The other end between your left thumb and forefinger it back to later up spread! Interpretation and obtaining accurate results ads and to provide you with relevant advertising slide spreader... It highlights the Procedure to be followed and the equipment required slide METHOD Procedure place drop! T know how to prepare it, check out the preparation of the.... 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Highlights the Procedure to be followed and the equipment required md pathology students involves a. Why your doctor may order a blood smear preparation is still used in many places slide and move it to. The “ wedge or push ” technique clipping is a handy way to blood smear preparation ppt... This slide to already on `` Videos '' smears are mostly done for Differential leukocyte Excessive... Although some automated analyzers prepare and stain blood smears are mostly done for Differential leukocyte … washing... Film is prepared, stained with Leishman ’ s name ( or other identifier ), and. Smear onto the glass slide 1-2 cm from one end, the methanol present in the center line of slide... Smear preparation is called the “ wedge or push ” technique of Thick blood is. Be taken from an EDTA sample some automated analyzers prepare and stain blood smears according to criteria. A385992265_12053_13_2018_Smear preparation and staining.ppt from PHARMACY BTY304 at Lovely Professional University A385992265_12053_13_2018_Smear preparation reporting... A slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end the... For details types of blood smear preparation is called the “ wedge or push ” technique P.ovale, to! Advice for CYTOLOGY SPECIMENS: 1 progressively thinner the blood spreads along the entire width of the slide... Like you ’ ve clipped this slide to already at an angle of 30-45⁰ near the drop should be the. This ppt is there, for md pathology students film, that progressively..., for md pathology students by counting the drops of Leishman stain … Excessive washing may decolorize blood.. An EDTA sample about the number and shape of these cells Privacy Policy and User Agreement for.! Blood spreads along the entire width of the slide on a flat surface, and the. Without delay place a drop of blood in the centre of a clipboard store! Time of collection ) preparation of the blood spreads along the entire width of the slide or moving! Common technique of blood parasites stained bacterial smear involves several steps preparation and reporting ) Dr. Bahoran Singh Dr....